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Continuous addition of sugars in 'fed batch' fermentation is done to
A
Degrade sewage
B
Produce methane
C
Obtain antibiotics
D
Purify enzymes
Detailed Solution
In a fed-batch culture, the substrate (sugar) is added to the bioreactor continuously or at intervals instead of being supplied all at once.
This keeps the sugar concentration low but steady, prevents substrate inhibition and prolongs the productive phase of the microbial culture.
As a result the cells keep producing their product, such as enzymes, in large amounts, which can then be recovered and purified in downstream processing.
Sewage degradation and methane production are carried out by mixed microbial populations in digesters, without controlled feeding of sugar.
The answer accepted in the key is 'purify enzymes'.
Note: several reference books give 'obtain antibiotics' for this question, since fed-batch culture is the standard method for antibiotics such as penicillin; the key of the source PDF is retained here.
This keeps the sugar concentration low but steady, prevents substrate inhibition and prolongs the productive phase of the microbial culture.
As a result the cells keep producing their product, such as enzymes, in large amounts, which can then be recovered and purified in downstream processing.
Sewage degradation and methane production are carried out by mixed microbial populations in digesters, without controlled feeding of sugar.
The answer accepted in the key is 'purify enzymes'.
Note: several reference books give 'obtain antibiotics' for this question, since fed-batch culture is the standard method for antibiotics such as penicillin; the key of the source PDF is retained here.