Looking for classes? Ksquare Career Institute, Bengaluru →
Plasmid has been used as vector because :
A
It is circular DNA which has capacity to join to eukaryotic DNA
B
It can move between prokaryotic and eukaryotic cells
C
Both ends show replication
D
It has antibiotic resistance gene
Detailed Solution
A plasmid is a small circular DNA that replicates independently in bacteria.
It can be cut with a restriction enzyme and a piece of foreign (e.g. eukaryotic) DNA can be joined into it with DNA ligase, forming recombinant DNA. When put back into a bacterium, the plasmid replicates and copies the foreign gene too.
Antibiotic resistance genes are useful as selectable markers, but the basic reason a plasmid is used as a vector is its ability to carry joined foreign DNA and replicate.
So, as per the answer key, plasmid is used as a vector because it is a circular DNA that can be joined to eukaryotic DNA.
It can be cut with a restriction enzyme and a piece of foreign (e.g. eukaryotic) DNA can be joined into it with DNA ligase, forming recombinant DNA. When put back into a bacterium, the plasmid replicates and copies the foreign gene too.
Antibiotic resistance genes are useful as selectable markers, but the basic reason a plasmid is used as a vector is its ability to carry joined foreign DNA and replicate.
So, as per the answer key, plasmid is used as a vector because it is a circular DNA that can be joined to eukaryotic DNA.