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Biolistics (gene-gun) is suitable for
A
DNA finger printing
B
Disarming pathogen vectors
C
Transformation of plant cells
D
Constructing recombinant DNA by joining with vectors
Detailed Solution
In biolistics (the gene-gun method), cells are bombarded with high-velocity micro-particles of gold or tungsten coated with DNA.
The particles penetrate the thick cell wall and deliver the DNA into the cell, so the method is suitable for plant cells.
It is a direct, vector-less method of introducing alien DNA into the host, i.e., of transformation.
DNA fingerprinting uses probes and electrophoresis, disarming of pathogen vectors is done by removing their disease-causing genes, and recombinant DNA is constructed using restriction enzymes and DNA ligase.
Hence biolistics is suitable for the transformation of plant cells.
The particles penetrate the thick cell wall and deliver the DNA into the cell, so the method is suitable for plant cells.
It is a direct, vector-less method of introducing alien DNA into the host, i.e., of transformation.
DNA fingerprinting uses probes and electrophoresis, disarming of pathogen vectors is done by removing their disease-causing genes, and recombinant DNA is constructed using restriction enzymes and DNA ligase.
Hence biolistics is suitable for the transformation of plant cells.