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Restriction endonucleases are enzymes which
A
Remove nucleotides from the ends of the DNA molecule
B
Make cuts at specific positions within the DNA molecule
C
Recognize a specific nucleotide sequence for binding of DNA ligase
D
Restrict the action of the enzyme DNA polymerase
Detailed Solution
Restriction enzymes belong to the class of enzymes called nucleases, which are of two kinds: exonucleases and endonucleases.
Exonucleases remove nucleotides from the ends of DNA.
Endonucleases make cuts at specific positions within the DNA.
Each restriction endonuclease inspects the length of the DNA, recognises a specific palindromic nucleotide sequence, binds to it and cuts each strand of the double helix at specific points in the sugar-phosphate backbone.
Hence restriction endonucleases make cuts at specific positions within the DNA molecule.
Exonucleases remove nucleotides from the ends of DNA.
Endonucleases make cuts at specific positions within the DNA.
Each restriction endonuclease inspects the length of the DNA, recognises a specific palindromic nucleotide sequence, binds to it and cuts each strand of the double helix at specific points in the sugar-phosphate backbone.
Hence restriction endonucleases make cuts at specific positions within the DNA molecule.