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Arrange the following steps of somatic hybridisation in a correct sequence. I. Digestion of cell walls. II. Isolation of naked protoplasts. III. Fusion of protoplasts to get hybrid protoplast. IV. Isolation of single cells from two different varieties of plants. V. Growing of hybrid protoplast to form a new plant. Choose the CORRECT answer from the options given below:
A
V, I, II, III, IV
B
IV, I, II, III, V
C
V, II, I, IV, III
D
IV, II, I, V, III
The answer is B.