An analysis of chromosomal DNA using the Southern hybridization technique does not use:

An analysis of chromosomal DNA using the Southern hybridization technique does not use:
A Electrophoresis
B Blotting
C Autoradiography
D PCR

Detailed Solution

Southern hybridisation involves digestion, gel electrophoresis, blotting on a membrane, hybridisation with a labelled probe and autoradiography. PCR is not a step in it.

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6 questions from this chapter have appeared across 5 exam years.

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Practise DNA Fingerprinting All 6 questions This chapter in 2014 AIPMT