In mitochondria, protons accumulate in the

In mitochondria, protons accumulate in the
A Intermembrane space
B Matrix
C Outer membrane
D Inner membrane

Detailed Solution

The electron transport system is located in the inner mitochondrial membrane.
As electrons pass through complexes I, III and IV, the energy released is used to pump protons ($H^+$) from the matrix across the inner membrane.
The protons accumulate in the intermembrane space (perimitochondrial space), creating a proton gradient across the inner membrane.
The protons then flow back to the matrix through the $F_0$ channel of ATP synthase, and this drives the synthesis of ATP by the $F_1$ headpiece.
Hence protons accumulate in the intermembrane space.

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