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Following are the steps involved in action of toxin in Bt. Cotton. A. The inactive toxin converted into active form due to alkaline pH of gut of insect. B. Bacillus thuringiensis produce crystals with toxic insecticidal proteins. C. The alkaline pH solubilises the crystals. D. The activated toxin binds to the surface of midgut cells, creates pores and causes death of the insect. E. The toxin proteins exist as inactive protoxins in bacteria. Choose the correct sequence of steps from the options given below:
Detailed Solution
Correct sequence: Bacillus thuringiensis produces crystals with toxic insecticidal proteins (B) → these toxin proteins exist as inactive protoxins in the bacteria (E) → the alkaline pH of the insect gut solubilises the crystals (C) → the inactive toxin is converted into active form due to the alkaline pH (A) → the activated toxin binds to midgut cells, creates pores, and kills the insect (D). So the order is B → E → C → A → D.