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Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain -
A
Lipase
B
Protease
C
Exonuclease
D
Endonuclease
Detailed Solution
Nucleases are enzymes that hydrolyse the phosphodiester bonds of nucleic acids.
Exonucleases remove nucleotides one at a time from the ends (the 5' or the 3' end) of a polynucleotide chain.
Endonucleases make cuts at specific positions within the polynucleotide chain, i.e. they hydrolyse the internal phosphodiester bonds.
Restriction enzymes are endonucleases that cut DNA at specific recognition sequences.
Lipases hydrolyse fats and proteases hydrolyse proteins.
So the enzyme that hydrolyses internal phosphodiester bonds is endonuclease.
Exonucleases remove nucleotides one at a time from the ends (the 5' or the 3' end) of a polynucleotide chain.
Endonucleases make cuts at specific positions within the polynucleotide chain, i.e. they hydrolyse the internal phosphodiester bonds.
Restriction enzymes are endonucleases that cut DNA at specific recognition sequences.
Lipases hydrolyse fats and proteases hydrolyse proteins.
So the enzyme that hydrolyses internal phosphodiester bonds is endonuclease.