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Biotechnology: Principles and Processes
Nucleases
2 questions from 2 papers, each with its answer.
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Concepts tested
- Endonucleases versus exonucleases
- Nucleases
All Questions
2019 Odisha 1 question
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An enzyme catalysing the removal of nucleotides from ends of DNA is:
2005 AIPMT 1 question
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Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain -Nucleases are enzymes that hydrolyse the phosphodiester bonds of nucleic acids.
Exonucleases remove nucleotides one at a time from the ends (the 5' or the 3' end) of a polynucleotide chain.
Endonucleases make cuts at specific positions within the polynucleotide chain, i.e. they hydrolyse the internal phosphodiester bonds.
Restriction enzymes are endonucleases that cut DNA at specific recognition sequences.
Lipases hydrolyse fats and proteases hydrolyse proteins.
So the enzyme that hydrolyses internal phosphodiester bonds is endonuclease.