pBR322 Cloning Vector - rop and ori Genes

2024 1 question

1. 1 Biotechnology: Principles and Processes Hard
   The following diagram showing restriction sites in $E.\ coli$ cloning vector $pBR322$. Find the role of 'X' and 'Y' genes:
   
   
   A The gene 'X' is for protein involved in replication of Plasmid and 'Y' for resistance to antibiotics. B Gene 'X' is responsible for recognition sites and 'Y' is responsible for antibiotic resistance. C The gene 'X' is responsible for resistance to antibiotics and 'Y' for protein involved in the replication of Plasmid. D The gene 'X' is responsible for controlling the copy number of the linked DNA and 'Y' for protein involved in the replication of Plasmid.
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