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Biotechnology: Principles and Processes
Selectable Markers - Blue-White Screening
1 question from 1 paper, each with its answer.
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Concepts tested
- Insertional Inactivation
All Questions
2025 1 question
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In the represented plasmid, an alien piece of DNA is inserted at the EcoRI site within the $\beta$-Galactosidase gene. Which of the following strategies will be chosen to select the recombinant colonies?Insertional inactivation of the $\beta$-galactosidase gene results in white recombinant colonies on chromogenic substrate (X-gal).
Insertion of recombinant foreign DNA into the coding region of $\beta$-galactosidase causes insertional inactivation. Non-recombinants retain active enzyme producing blue colonies with X-gal, while recombinants cannot produce active enzyme and appear white.
