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Biotechnology: Principles and Processes
pBR322 vector - restriction sites within resistance genes
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- pBR322 vector - restriction sites within resistance genes
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2023 Manipur 1 question
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Ligation of foreign DNA at which of the following site will result in loss of tetracyclin resistance of pBR322:The $BamHI$ restriction site lies within the tetracycline resistance ($tet^R$) gene of pBR322, so ligating foreign DNA at this site disrupts (inactivates) the $tet^R$ gene, resulting in loss of tetracycline resistance.