Cloning Vectors – Insertional Inactivation

2021 1 question

1. 1 Biotechnology: Principles and Processes Hard
   Plasmid pBR322 has PstI restriction enzyme site within gene $amp^R$ that confers ampicillin resistance. If this enzyme is used for inserting a gene for $\beta$-galactoside production and the recombinant plasmid is inserted in an E.coli strain
   A The transformed cells will have the ability to resist ampicillin as well as produce $\beta$-galactosidase. B It will lead to lysis of host cell. C It will be able to produce a novel protein with dual ability. D It will not be able to confer ampicillin resistance to host cell.
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