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Biotechnology: Principles and Processes
Gel Electrophoresis
5 questions from 5 papers, each with its answer.
- questions
- 5
- papers
- 5
- years asked, 2017–2026
- 4 of 10
- of Biotechnology: Principles and Processes
- 3.79%
Appears in
Concepts tested
- Gel electrophoresis 2
- Agarose Gel Separation
- gel-electrophoresis-size
- Separation of DNA fragments
All Questions
2025 1 question
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Given below are two statements:
Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.
Statement II: Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel.
In the light of the above statements, choose the most appropriate answer from the options given below:Both statements regarding agarose gel electrophoresis and elution are correct.
DNA fragments resolved on agarose gel are cut out and extracted (elution) to construct rDNA vectors (Statement I is correct). Negatively charged DNA migrates toward the positive anode; smaller fragments encounter lower sieving resistance and travel farthest toward the anode, while large fragments remain near the cathode wells (Statement II is correct).
2024 Re-NEET 1 question
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Identify the incorrect statement related to gel electrophoresis.DNA fragments separated by gel electrophoresis cannot be seen directly; the gel must first be stained with a compound such as ethidium bromide, and the DNA bands are then visualised under UV radiation. Pure DNA fragments cannot be seen in visible light without staining, so the statement in option (a) is incorrect.
2019 Odisha 1 question
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Given below are four statements pertaining to separation of DNA fragments using gel electrophoresis. Identify the incorrect statements. (a) DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal (b) DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA. (c) Smaller the size of DNA fragment larger is the distance it travels through it. (d) Pure DNA can be visualized directly by exposing UV radiation. Choose correct answer from the options given below
2017 1 question
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What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?
Smaller fragments migrate faster through the gel matrix.
During gel electrophoresis, DNA fragments separate (resolve) according to their size through the sieving effect provided by the agarose gel; smaller fragments move farther.
2013 NEET 1 question
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DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by:DNA fragments are negatively charged and are separated by size using gel electrophoresis.