2024 Re-NEET Biotechnology: Principles and ProcessesGel Electrophoresis Medium Question 2 of 5 in Gel Electrophoresis

Identify the incorrect statement related to gel electrophoresis.

A Separated DNA fragments can be directly seen under UV radiation
B Separated DNA can be extracted from gel piece
C Fragment of DNA moves toward anode
D Sieving effect of agarose gel helps in separation of DNA fragments

The answer is A.

Solution

DNA fragments separated by gel electrophoresis cannot be seen directly; the gel must first be stained with a compound such as ethidium bromide, and the DNA bands are then visualised under UV radiation. Pure DNA fragments cannot be seen in visible light without staining, so the statement in option (a) is incorrect.

Concept Gel electrophoresis

Gel Electrophoresis in past papers

5 questions from this chapter have appeared across 5 exam years.