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Given below are two statements:
Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.
Statement II: Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel.
In the light of the above statements, choose the most appropriate answer from the options given below:
Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.
Statement II: Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel.
In the light of the above statements, choose the most appropriate answer from the options given below:
Explanation
Both statements regarding agarose gel electrophoresis and elution are correct.
Detailed Solution
DNA fragments resolved on agarose gel are cut out and extracted (elution) to construct rDNA vectors (Statement I is correct). Negatively charged DNA migrates toward the positive anode; smaller fragments encounter lower sieving resistance and travel farthest toward the anode, while large fragments remain near the cathode wells (Statement II is correct).