2025 Biotechnology: Principles and ProcessesGel Electrophoresis Easy Question 1 of 5 in Gel Electrophoresis

Given below are two statements:
Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.
Statement II: Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel.
In the light of the above statements, choose the most appropriate answer from the options given below:

A Both statement I and statement II are correct
B Both statement I and statement II are incorrect
C Statement I is correct but statement II is incorrect
D Statement I is incorrect but statement II is correct

The answer is A.

Solution

Both statements regarding agarose gel electrophoresis and elution are correct.

DNA fragments resolved on agarose gel are cut out and extracted (elution) to construct rDNA vectors (Statement I is correct). Negatively charged DNA migrates toward the positive anode; smaller fragments encounter lower sieving resistance and travel farthest toward the anode, while large fragments remain near the cathode wells (Statement II is correct).

Concept Agarose Gel Separation

Gel Electrophoresis in past papers

5 questions from this chapter have appeared across 5 exam years.