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Biotechnology: Principles and Processes
Tools of Recombinant DNA Technology
5 questions from 5 papers, each with its answer.
- questions
- 5
- papers
- 5
- years asked, 2017–2026
- 2 of 10
- of Biotechnology: Principles and Processes
- 3.79%
Appears in
Concepts tested
- DNA ligase as molecular glue
- Gene Cloning Enzymes
- Microbes used in genetic engineering
- organisms used in biotechnology
- Role of DNA ligase
All Questions
2025 1 question
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Which of the following enzyme(s) are NOT essential for gene cloning?
A. Restriction enzymes
B. DNA ligase
C. DNA mutase
D. DNA recombinase
E. DNA polymerase
Choose the correct answer from the options given below:DNA mutase and DNA recombinase are not required tools in recombinant DNA cloning vectors.
Core recombinant cloning enzymes comprise restriction endonucleases (cutting), DNA ligase (joining), and DNA polymerases (synthesis/PCR). DNA mutase and recombinase are non-essential/irrelevant.
2020 1 question
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Match the organism with its use in biotechnology.
Pick the matching item from Column II for each row.
Column I
- A. Bacillus thuringiensis
- B. Thermus aquaticus
- C. Agrobacterium tumefaciens
- D. Salmonella typhimurium
Column II
- I. Cloning vector
- II. Construction of first rDNA molecule
- III. DNA polymerase
- IV. Cry proteins
Correct answer: A → IV, B → III, C → I, D → II
2008 AIPMT 1 question
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The linking of antibiotic resistance gene with the plasmid vector became possible with:The first recombinant DNA was constructed by Stanley Cohen and Herbert Boyer in 1972.
They cut a piece of DNA carrying an antibiotic resistance gene from a plasmid of Salmonella typhimurium using a restriction enzyme (the molecular scissors).
The cut piece of DNA was then linked with the plasmid DNA, which acted as the vector.
This linking of the antibiotic resistance gene with the plasmid vector became possible with the enzyme DNA ligase, which acts on cut DNA molecules and joins their ends by forming phosphodiester bonds.
Endonucleases cut DNA at specific sites within the molecule, exonucleases remove nucleotides from the ends, and DNA polymerase synthesises new DNA; none of these joins two DNA fragments.
So the enzyme is DNA ligase.
2006 AIPMT-PRE 1 question
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Two microbes found to be very useful in genetic engineering are -Escherichia coli is the most widely used host in genetic engineering. Its plasmids, such as pBR322, are used as cloning vectors, its restriction enzyme EcoRI is used to cut DNA, and it multiplies rapidly to produce the cloned gene or its product (for example human insulin).
Agrobacterium tumefaciens is a soil bacterium that causes crown gall disease in dicot plants. It naturally transfers a piece of DNA, the T-DNA of its Ti (tumour inducing) plasmid, into the plant genome.
The Ti plasmid has been modified into a cloning vector that is no longer pathogenic but can still deliver genes of interest into plants; Agrobacterium is therefore called the natural genetic engineer of plants.
Caenorhabditis elegans is a nematode, not a microbe used as a tool in this sense.
Vibrio cholerae and Diplococcus are not standard tools of genetic engineering.
So the two very useful microbes are Escherichia coli and Agrobacterium tumefaciens.
2002 AIPMT 1 question
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Which of the following enzymes are used to join bits of DNA : -DNA ligase joins two DNA fragments by forming a phosphodiester bond between the 3'-OH end of one fragment and the 5'-phosphate end of the other.
It is used in genetic engineering to join the gene of interest with the vector DNA, and in the cell to join Okazaki fragments during replication.
Primase makes RNA primers, DNA polymerase synthesises new DNA, and endonucleases cut DNA.
So ligase is used to join bits of DNA ('molecular glue').