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Biotechnology: Principles and Processes
Cloning Vectors: Ti Plasmid
1 question from 1 paper, each with its answer.
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Concepts tested
- Ti plasmid of Agrobacterium tumefaciens
All Questions
2004 AIPMT 1 question
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The Ti plasmid is often used for making transgenic plants. This plasmid is found inTi (tumour-inducing) plasmid is found in the soil bacterium Agrobacterium tumefaciens, which causes crown gall in many dicot plants.
A part of this plasmid, the T-DNA, is naturally transferred into the plant genome, where it causes tumours.
Scientists remove the tumour-causing genes (disarming the plasmid) and insert the desired gene into the T-DNA. The modified Ti plasmid then acts as a vector to carry useful genes into plants, producing transgenic plants.
Yeast carries the 2 $\mu m$ plasmid, which is different; Rhizobium and Azotobacter are nitrogen-fixing bacteria.
So the Ti plasmid is found in Agrobacterium.