Processes of Recombinant DNA Technology – PCR

2021 3 questions

1. 1 Biotechnology: Principles and Processes Medium
   Which of the following is not an application of PCR (Polymerase Chain Reaction)?
   A Gene amplification B Purification of isolated protein C Detection of gene mutation D Molecular diagnosis
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2. 2 Biotechnology: Principles and Processes Easy
   Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?
   A Denaturation, Extension, Annealing B Extension, Denaturation, Annealing C Annealing, Denaturation, Extension D Denaturation, Annealing, Extension
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3. 3 Biotechnology: Principles and Processes Medium
   During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
   A Extension B Denaturation C Ligation D Annealing
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