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Which of the following enzymes are used to join bits of DNA : -
A
Ligase
B
Primase
C
DNA polymerase
D
Endonuclease
Detailed Solution
DNA ligase joins two DNA fragments by forming a phosphodiester bond between the 3'-OH end of one fragment and the 5'-phosphate end of the other.
It is used in genetic engineering to join the gene of interest with the vector DNA, and in the cell to join Okazaki fragments during replication.
Primase makes RNA primers, DNA polymerase synthesises new DNA, and endonucleases cut DNA.
So ligase is used to join bits of DNA ('molecular glue').
It is used in genetic engineering to join the gene of interest with the vector DNA, and in the cell to join Okazaki fragments during replication.
Primase makes RNA primers, DNA polymerase synthesises new DNA, and endonucleases cut DNA.
So ligase is used to join bits of DNA ('molecular glue').