2025 Biotechnology: Principles and ProcessesTools of Recombinant DNA Technology Easy Question 1 of 5 in Tools of Recombinant DNA Technology

Which of the following enzyme(s) are NOT essential for gene cloning?
A. Restriction enzymes
B. DNA ligase
C. DNA mutase
D. DNA recombinase
E. DNA polymerase
Choose the correct answer from the options given below:

A C and D only
B A and B only
C D and E only
D B and C only

The answer is A.

Solution

DNA mutase and DNA recombinase are not required tools in recombinant DNA cloning vectors.

Core recombinant cloning enzymes comprise restriction endonucleases (cutting), DNA ligase (joining), and DNA polymerases (synthesis/PCR). DNA mutase and recombinase are non-essential/irrelevant.

Concept Gene Cloning Enzymes

Tools of Recombinant DNA Technology in past papers

5 questions from this chapter have appeared across 5 exam years.