Looking for classes? Ksquare Career Institute, Bengaluru →
Following are the steps involved in the process of PCR. A. Annealing B. Amplification (~1 billion times) C. Denaturation D. Treatment with Taq polymerase and deoxynucleotides E. Extension. Choose the correct sequence of steps of PCR from the options given below:
A
C → A → D → E → B
B
A → B → E → D → C
C
A → C → E → D → B
D
D → B → E → C → A
Detailed Solution
PCR proceeds through: Denaturation of the DNA strands (C) → Annealing of primers to the single-stranded DNA (A) → Treatment with Taq polymerase and deoxynucleotides for primer extension (D) → Extension of the new strand (E) → Amplification of the DNA (~1 billion times) after repeated cycles (B). Order: C → A → D → E → B.