Looking for classes? Ksquare Career Institute, Bengaluru →
Polymerase Chain Reaction (PCR)
-
Polymerase chain reaction (PCR) amplifies DNA following the equation.A $N^{2}$B $2^{n}$C $2n+1$D $2N^{2}$
DNA amplifies exponentially as $2^n$ in PCR.
In PCR, each cycle doubles the amount of target DNA. Thus, after $n$ cycles, DNA molecules increase by a factor of $2^{n}$. -
Following are the steps involved in the process of PCR. A. Annealing B. Amplification (~1 billion times) C. Denaturation D. Treatment with Taq polymerase and deoxynucleotides E. Extension. Choose the correct sequence of steps of PCR from the options given below:A C → A → D → E → BB A → B → E → D → CC A → C → E → D → BD D → B → E → C → APCR proceeds through: Denaturation of the DNA strands (C) → Annealing of primers to the single-stranded DNA (A) → Treatment with Taq polymerase and deoxynucleotides for primer extension (D) → Extension of the new strand (E) → Amplification of the DNA (~1 billion times) after repeated cycles (B). Order: C → A → D → E → B.
