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Biotechnology: Principles and Processes
Polymerase Chain Reaction (PCR)
3 questions, each with its answer.
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Polymerase chain reaction (PCR) amplifies DNA following the equation.
DNA amplifies exponentially as $2^n$ in PCR.
In PCR, each cycle doubles the amount of target DNA. Thus, after $n$ cycles, DNA molecules increase by a factor of $2^{n}$. -
Following are the steps involved in the process of PCR. A. Annealing B. Amplification (~1 billion times) C. Denaturation D. Treatment with Taq polymerase and deoxynucleotides E. Extension. Choose the correct sequence of steps of PCR from the options given below:PCR proceeds through: Denaturation of the DNA strands (C) → Annealing of primers to the single-stranded DNA (A) → Treatment with Taq polymerase and deoxynucleotides for primer extension (D) → Extension of the new strand (E) → Amplification of the DNA (~1 billion times) after repeated cycles (B). Order: C → A → D → E → B.
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The correct order of steps in Polymerase Chain Reaction (PCR) is
Each PCR cycle: denaturation → annealing → extension.
PCR is used for making multiple copies of a gene (or DNA) of interest in vitro. Each cycle has three steps: (i) Denaturation, (ii) Primer annealing, (iii) Extension of primer.
That is every question in Polymerase Chain Reaction (PCR).
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