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Biotechnology: Principles and Processes

Tools and vectors in genetic engineering

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1 2023 Manipur Biotechnology: Principles and Processes Medium

Match List-I with List-II.

Pick the matching item from Column II for each row.
Column I
- A. Kanamycin Match for A
- B. ClaI Match for B
- C. Disarmed retroviruses Match for C
- D. Kanamycin R gene Match for D
Column II
- I. Delivers genes into animal cells
- II. Selectable marker
- III. Restriction site
- IV. Antibiotic resistance
Correct answer: A → IV, B → III, C → I, D → II

Kanamycin is an antibiotic used as a selection agent, related to antibiotic resistance (A-IV); ClaI is a restriction enzyme with a specific restriction site (B-III); Disarmed retroviruses are used to deliver genes into animal cells (C-I); the Kanamycin resistance gene ($Kan^R$) acts as a selectable marker (D-II).

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