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Which of the following cuts the DNA from specific places : -
A
Restriction endonuclease (EcoRI)
B
Ligase
C
Exonuclease
D
Alkaline phosphatase
Detailed Solution
Restriction endonucleases recognise specific (palindromic) base sequences and cut DNA within or near those sites.
EcoRI recognises 5'-GAATTC-3' and cuts between G and A on each strand, giving sticky ends.
Ligase joins DNA, exonucleases remove nucleotides from the ends of DNA (not at specific internal sites), and alkaline phosphatase removes phosphate groups.
So restriction endonuclease (EcoRI) cuts DNA at specific places.
EcoRI recognises 5'-GAATTC-3' and cuts between G and A on each strand, giving sticky ends.
Ligase joins DNA, exonucleases remove nucleotides from the ends of DNA (not at specific internal sites), and alkaline phosphatase removes phosphate groups.
So restriction endonuclease (EcoRI) cuts DNA at specific places.