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ELISA is used to detect viruses, where :-
A
Southern blotting is done
B
Alkaline phosphatase is the key reagent
C
Catalase is the key reagent
D
DNA-probes are required
Detailed Solution
ELISA (Enzyme Linked Immuno-Sorbent Assay) is based on the antigen-antibody reaction. It detects the presence of viral antigens or of antibodies made against the virus (e.g. for HIV).
The antibody used is linked to an enzyme. After binding, a colourless substrate is added; the enzyme converts it into a coloured product, and the colour shows a positive result.
The enzymes commonly linked are alkaline phosphatase and horseradish peroxidase, so the enzyme (alkaline phosphatase) is the key reagent.
Southern blotting and DNA probes are used in DNA-based techniques, not in ELISA; catalase is not the enzyme used.
So in ELISA, alkaline phosphatase is the key reagent.
The antibody used is linked to an enzyme. After binding, a colourless substrate is added; the enzyme converts it into a coloured product, and the colour shows a positive result.
The enzymes commonly linked are alkaline phosphatase and horseradish peroxidase, so the enzyme (alkaline phosphatase) is the key reagent.
Southern blotting and DNA probes are used in DNA-based techniques, not in ELISA; catalase is not the enzyme used.
So in ELISA, alkaline phosphatase is the key reagent.