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ELISA is used to detect viruses where the key reagent is : -
A
Alkaline phosphatase
B
Catalase
C
DNA probe
D
RNase
Detailed Solution
ELISA (Enzyme Linked Immuno-Sorbent Assay) is based on the antigen-antibody reaction. It detects viral antigens or the antibodies formed against the virus (e.g. HIV).
The detecting antibody is linked to an enzyme. After washing, a colourless substrate is added and the enzyme converts it into a coloured product, showing a positive result.
The enzymes commonly used are alkaline phosphatase and horseradish peroxidase.
DNA probes are used in hybridisation tests, not ELISA.
So in ELISA the key reagent is alkaline phosphatase.
The detecting antibody is linked to an enzyme. After washing, a colourless substrate is added and the enzyme converts it into a coloured product, showing a positive result.
The enzymes commonly used are alkaline phosphatase and horseradish peroxidase.
DNA probes are used in hybridisation tests, not ELISA.
So in ELISA the key reagent is alkaline phosphatase.