2021 Biotechnology and its ApplicationsMolecular Diagnosis Hard Question 2 of 5 in Molecular Diagnosis

Now a days it is possible to detect the mutated gene causing cancer by allowing radioactive probe to hybridise its complimentary DNA in a clone of cells, followed by its detection using autoradiography because:

A Mutated gene completely and clearly appears on a photographic film.
B Mutated gene does not appear on a photographic film as the prober has no complimentarity with it.
C Mutated gene does not appear on a photographic film as the probe has complimentarity with it.
D Mutated gene partially appears on a photographic film.

The answer is B.

Solution

Concept probe hybridisation and autoradiography

Molecular Diagnosis in past papers

5 questions from this chapter have appeared across 4 exam years.