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Molecular Diagnosis
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Which one of the following techniques does not serve the purpose of early diagnosis of a disease for its early treatment?A Enzyme Linked Immuno-Sorbent Assay (ELISA) techniqueB Recombinant DNA TechnologyC Serum and Urine analysisD Polymerase Chain Reaction (PCR) technique
PCR, ELISA and rDNA technology detect infection early; serum and urine analysis does not.
Of these, serum and urine analysis does not fulfil the purpose of early diagnosis of a disease. In early stages of infections, when the disease symptoms are not visible, amplification of pathogenic nucleic acid can be done by PCR, detection of antibodies produced by host against pathogen by ELISA and rDNA technology can serve the purpose of early diagnosis. -
Now a days it is possible to detect the mutated gene causing cancer by allowing radioactive probe to hybridise its complimentary DNA in a clone of cells, followed by its detection using autoradiography because:A Mutated gene completely and clearly appears on a photographic film.B Mutated gene does not appear on a photographic film as the prober has no complimentarity with it.C Mutated gene does not appear on a photographic film as the probe has complimentarity with it.D Mutated gene partially appears on a photographic film.
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For effective treatment of the disease, early diagnosis and understanding its pathophysiology is very important. Which of the following molecular diagnostic techniques is very useful for early detection?A Southern Blotting TechniqueB ELISA TechniqueC Hybridization TechniqueD Western Blotting Technique