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The figure below is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly identifies its certain component(s)?
A
$amp^R$, $tet^R$ – antibiotic resistance genes
B
ori – original restriction enzyme
C
rop – reduced osmotic pressure
D
Hind III, EcoRI – selectable markers
Detailed Solution
In pBR322, $amp^R$ and $tet^R$ are genes for resistance to ampicillin and tetracycline; they serve as selectable markers.
ori is the origin of replication, rop codes for proteins involved in the replication of the plasmid, and Hind III and EcoRI are restriction sites.
ori is the origin of replication, rop codes for proteins involved in the replication of the plasmid, and Hind III and EcoRI are restriction sites.