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Which of the following are used in gene cloning?
A
Nucleoids
B
Lomasomes
C
Mesosomes
D
Plasmids
Detailed Solution
Gene cloning needs a vector, a DNA molecule that can carry the foreign gene into a host cell and replicate there.
Plasmids are small, circular, extra-chromosomal DNA molecules of bacteria that replicate independently of the chromosomal DNA; they are the most commonly used cloning vectors (e.g., pBR322).
A nucleoid is the main genetic material of a bacterium, a mesosome is an infolding of the bacterial plasma membrane, and lomasomes are membranous structures found in fungal cells; none of them is used as a vector.
Hence plasmids are used in gene cloning.
Plasmids are small, circular, extra-chromosomal DNA molecules of bacteria that replicate independently of the chromosomal DNA; they are the most commonly used cloning vectors (e.g., pBR322).
A nucleoid is the main genetic material of a bacterium, a mesosome is an infolding of the bacterial plasma membrane, and lomasomes are membranous structures found in fungal cells; none of them is used as a vector.
Hence plasmids are used in gene cloning.