2024 Re-NEET Biotechnology: Principles and ProcessespBR322 cloning vector - restriction sites Medium Question 25 of 132 in Biotechnology: Principles and Processes

Select the restriction endonuclease enzymes whose restriction sites are present for the tetracycline resistance ($tet^R$) gene in the pBR322 cloning vector.

A Bam HI and Sal I
B Sal I and Pst I
C Pst I and Pvu I
D Pvu I and Bam HI

The answer is A.

Solution

In the pBR322 vector, the tetracycline resistance ($tet^R$) gene contains unique restriction sites for Bam HI and Sal I, which are commonly used for inserting foreign DNA at this locus.

Concept pBR322 cloning vector - restriction sites

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132 questions from this chapter have appeared across 26 exam years.