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Which one is a true statement regarding DNA polymerase used in PCR?
A
It remains active at high temperature
B
It is used to ligate introduced DNA in recipient cells
C
It serves as a selectable marker
D
It is isolated from a virus
Detailed Solution
PCR uses a thermostable DNA polymerase (Taq polymerase) isolated from the bacterium Thermus aquaticus.
It remains active during the high-temperature denaturation step, so it need not be added again in each cycle.
Ligation is done by DNA ligase, and the enzyme is from a bacterium, not a virus.
It remains active during the high-temperature denaturation step, so it need not be added again in each cycle.
Ligation is done by DNA ligase, and the enzyme is from a bacterium, not a virus.