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The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of:
A
Inactivation of glycosidase enzyme in recombinant bacteria
B
Non-recombinant bacteria containing beta-galactosidase
C
Insertional inactivation of alpha-galactosidase in non-recombinant bacteria
D
Insertional inactivation of alpha-galactosidase in recombinant bacteria
Detailed Solution
Non-recombinant bacteria have an intact beta-galactosidase gene, so they act on the chromogenic substrate and give blue colonies.
In recombinants the insert inactivates this gene (insertional inactivation), so their colonies stay white.
In recombinants the insert inactivates this gene (insertional inactivation), so their colonies stay white.