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Which of the following graphs depicts the effect of substrate concentration on velocity of enzyme catalysed reaction? [add image: four graph options (a)-(d)]
A
[add image: velocity rises and plateaus at Vmax - hyperbolic curve]
B
[add image: velocity increases linearly without limit]
C
[add image: velocity rises then falls after a peak]
D
[add image: velocity decreases continuously]
Detailed Solution
As substrate concentration increases, the velocity of an enzyme-catalysed reaction rises initially and then plateaus at $V_{max}$, since all enzyme molecules become saturated with substrate and no free enzyme molecules remain to bind additional substrate. This gives a hyperbolic (rectangular hyperbola) curve.