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Enzyme kinetics - effect of substrate concentration
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Which of the following graphs depicts the effect of substrate concentration on velocity of enzyme catalysed reaction? [add image: four graph options (a)-(d)]A [add image: velocity rises and plateaus at Vmax - hyperbolic curve]B [add image: velocity increases linearly without limit]C [add image: velocity rises then falls after a peak]D [add image: velocity decreases continuously]As substrate concentration increases, the velocity of an enzyme-catalysed reaction rises initially and then plateaus at $V_{max}$, since all enzyme molecules become saturated with substrate and no free enzyme molecules remain to bind additional substrate. This gives a hyperbolic (rectangular hyperbola) curve.