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Regulation of gene expression
Concepts tested here
- Lac operon 2
All Questions
2010 AIPMT-MAINS 1 question
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The lac operon consists ofThe lac operon is a polycistronic unit in which the structural genes are regulated by a common promoter and regulatory gene.
It has one regulatory gene, the i gene (i for inhibitor), which codes for the repressor.
It has three structural genes: z codes for β-galactosidase, which hydrolyses lactose into galactose and glucose; y codes for permease, which increases the permeability of the cell to β-galactosides; a codes for transacetylase.
Hence the lac operon consists of one regulatory gene and three structural genes.
2010 AIPMT-PRE 1 question
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Select the two correct statements out of the four (a–d) given below about lac operon.
(a) Glucose or galactose may bind with the repressor and inactivate it
(b) In the absence of lactose the repressor binds with the operator region
(c) The z-gene codes for permease
(d) This was elucidated by Francois Jacob and Jacque Monod
The correct statements are(a) is wrong: the inducer of the lac operon is lactose (allolactose); it binds to the repressor and inactivates it. Glucose and galactose cannot act as inducers.
(b) is correct: in the absence of the inducer (lactose), the repressor protein made by the i gene binds to the operator region and prevents RNA polymerase from transcribing the operon.
(c) is wrong: the z gene codes for β-galactosidase; permease is coded by the y gene and transacetylase by the a gene.
(d) is correct: the lac operon was elucidated by the geneticist Francois Jacob and the biochemist Jacque Monod.
Hence the correct statements are (b) and (d).
