Pest resistant plants

The nematode $Meloidogyne\ incognita$ infects the roots of tobacco plants and reduces the yield.
To prevent this, nematode-specific genes were introduced into the host plant using $Agrobacterium$ vectors.
The introduced DNA was such that it produced both sense and anti-sense RNA in the host cells.
These two RNAs, being complementary to each other, formed a double-stranded RNA (dsRNA) that initiated RNA interference (RNAi).
RNAi silenced the specific mRNA of the nematode, so the parasite could not survive in the transgenic host.
A toxic protein is the strategy of Bt crops, not of this nematode-resistant tobacco.
Hence the introduced DNA produced both sense and anti-sense RNA.

Silencing of a specific mRNA by a complementary double-stranded RNA is called RNA interference (RNAi).
The nematode $Meloidogyne\ incognita$ infects the roots of tobacco plants and reduces the yield.
Using $Agrobacterium$ vectors, nematode-specific genes were introduced into the host so that both sense and anti-sense RNA were produced; these formed dsRNA that silenced the specific mRNA of the nematode.
The parasite could not survive in such a transgenic host.
Resistance to bollworms was obtained with Bt toxin genes (cry genes), not by mRNA silencing.
Hence mRNA silencing has been used to produce plants resistant to nematodes.

The nematode $Meloidogyne\ incognita$ infects the roots of tobacco plants and greatly reduces the yield.
Using $Agrobacterium$ vectors, nematode-specific genes were introduced into the host plant in such a way that both sense and anti-sense RNA were produced in the host cells.
These complementary RNAs formed double-stranded RNA, which initiated RNA interference (RNAi) and silenced the specific mRNA of the nematode.
The parasite could not survive in the transgenic host, so the plant was protected.
Resistance to insects was obtained by a different method, the Bt toxin genes.
Hence RNA interference has been used to develop plants resistant to nematodes.