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Transgenic animals and rDNA technology
Concepts tested here
- Steps of recombinant DNA technology
All Questions
2011 AIPMT-MAINS 1 question
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Read the following four statements (A–D) about certain mistakes in two of them.
(A) The first transgenic buffalo Rosie produced milk which was human alpha-lactalbumin enriched
(B) Restriction enzymes are used in isolation of DNA from other macro-molecules
(C) Downstream processing is one of the steps of R-DNA technology
(D) Disarmed pathogen vectors are also used in transfer of R-DNA into the host
Which are the two statements having mistakes?(A) has a mistake: Rosie was the first transgenic cow, not a buffalo; her milk contained human alpha-lactalbumin (2.4 g per litre).
(B) has a mistake: restriction enzymes cut DNA at specific recognition sequences. DNA is isolated from other macromolecules by using enzymes such as lysozyme, cellulase or chitinase, ribonuclease and protease, followed by precipitation with chilled ethanol.
(C) is correct: downstream processing (separation and purification of the product) is one of the steps of recombinant DNA technology.
(D) is correct: disarmed pathogen vectors such as the Ti plasmid of $Agrobacterium$ and retroviruses are used to transfer recombinant DNA into the host.
Hence the two statements with mistakes are (A) and (B).