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Biology
Biotechnology: Principles and Processes
132 questions from 36 papers, each with its answer.
- 132 questions
- 36 papers
- 3.74% of Biology
Taq polymerase in PCR
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Thermostable DNA polymerase used in PCR was isolated from:The thermostable DNA polymerase (Taq polymerase) used in PCR was isolated from the thermophilic bacterium Thermus aquaticus, which allows it to withstand the repeated high-temperature denaturation steps of the PCR cycle.
pBR322 vector - restriction sites within resistance genes
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Ligation of foreign DNA at which of the following site will result in loss of tetracyclin resistance of pBR322:The $BamHI$ restriction site lies within the tetracycline resistance ($tet^R$) gene of pBR322, so ligating foreign DNA at this site disrupts (inactivates) the $tet^R$ gene, resulting in loss of tetracycline resistance.
Agrobacterium tumefaciens as a gene delivery vector
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Which of the following statement is incorrect about Agrobacterium tumifaciens?Agrobacterium tumefaciens is used primarily as a vector for delivering genes of interest into plant (eukaryotic) host cells, not prokaryotic cells — so the statement that it is used for both prokaryotic and eukaryotic hosts is incorrect.
Tools and vectors in genetic engineering
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Match List-I with List-II.
Pick the matching item from Column II for each row.
Column I
- A. Kanamycin
- B. ClaI
- C. Disarmed retroviruses
- D. Kanamycin R gene
Column II
- I. Delivers genes into animal cells
- II. Selectable marker
- III. Restriction site
- IV. Antibiotic resistance
Correct answer: A → IV, B → III, C → I, D → II
Kanamycin is an antibiotic used as a selection agent, related to antibiotic resistance (A-IV); ClaI is a restriction enzyme with a specific restriction site (B-III); Disarmed retroviruses are used to deliver genes into animal cells (C-I); the Kanamycin resistance gene ($Kan^R$) acts as a selectable marker (D-II).
Restriction enzymes as molecular scissors
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Which of the following can act as molecular scissors?Restriction enzymes (restriction endonucleases) cut DNA at specific recognition sequences, acting as 'molecular scissors', while DNA ligase joins DNA fragments together.
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