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Biology
Biotechnology: Principles and Processes
132 questions from 36 papers, each with its answer.
- 132 questions
- 36 papers
- 3.74% of Biology
Restriction Enzymes and Recognition Sequences
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$Hind$ II always cuts DNA molecules at a particular point called recognition sequence and it consists of:$Hind$ II was the first restriction endonuclease isolated whose action depended on a specific DNA nucleotide sequence. It always cuts DNA at a particular point by recognising a specific sequence of six base pairs (GTPy↓PuAC), known as its recognition sequence. So the recognition sequence consists of 6 bp.
Fate of Foreign DNA in a Recipient Organism
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What is the fate of piece of DNA carrying only gene of interest which is transferred into an alien organism? A. The piece of DNA would be able to multiply itself independently in the progeny cells of the organisms. B. It may get integrated into the genome of the recipient. C. It may multiply and be inherited along with the host DNA. D. The alien piece of DNA is not an integrated part of chromosome. E. It shows ability to replicate. Choose the correct answer from the options given below:A piece of DNA carrying only the gene of interest (without an origin of replication) cannot multiply independently in the alien organism. It may get integrated into the genome of the recipient (B), and then it may multiply and be inherited along with the host DNA (C). So B and C only are correct.
Somatic Hybridization Technique
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Which of the following are fused in somatic hybridization involving two varieties of plants?In somatic hybridisation, protoplasts (cells whose cell walls have been removed) from two different varieties of plants are fused to form hybrid protoplasts. These are then grown to form a new plant (somatic hybrid), e.g. pomato.
pBR322 Cloning Vector - rop and ori Genes
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The following diagram showing restriction sites in $E.\ coli$ cloning vector $pBR322$. Find the role of 'X' and 'Y' genes:
'X' represents ori (origin of replication), which is responsible for controlling the copy number of the linked DNA.
'Y' represents rop, which codes for the proteins involved in the replication of the plasmid.
Bioreactors and Large-scale Production
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Which of the following statements is incorrect?Bioreactors are used to produce large quantities (100–1000 litres) of bacterial cultures, not small-scale cultures – so this statement is incorrect. Bioreactors do have an agitator system, an oxygen delivery system and a foam control system, they provide optimal growth conditions (temperature, pH, substrate, salts, vitamins, oxygen), and the most commonly used ones are of the stirring type.
Ti Plasmid of Agrobacterium tumefaciens
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The \"Ti plasmid\"" of $Agrobacterium\ tumefaciens$ stands for"The Ti plasmid of $Agrobacterium\ tumefaciens$ is the tumour inducing plasmid. Its T-DNA causes tumours in several dicot plants by transforming normal plant cells into tumour cells; it has now been modified into a cloning vector.
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