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Biology
Biotechnology: Principles and Processes
132 questions from 36 papers, each with its answer.
- 132 questions
- 36 papers
- 3.74% of Biology
Gel Electrophoresis
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What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?
Smaller fragments migrate faster through the gel matrix.
During gel electrophoresis, DNA fragments separate (resolve) according to their size through the sieving effect provided by the agarose gel; smaller fragments move farther.
Cloning vectors - selectable markers
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A gene whose expression helps to identify transformed cell is known as
Selectable markers identify transformants.
In recombinant DNA technology, selectable markers help in identifying and eliminating non-transformants and selectively permitting the growth of transformants.
Downstream processing
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The process of separation and purification of expressed protein before marketing is called
Separation and purification = downstream processing.
The biosynthetic stage for synthesis of product in recombinant DNA technology is called upstream processing; after it, the product is subjected to a series of processes including separation and purification, collectively referred to as downstream processing.
Gel electrophoresis – charge on DNA
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DNA fragments are
Phosphate backbone makes DNA negatively charged.
DNA fragments are negatively charged because of phosphate groups.
Gel electrophoresis – visualisation
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The DNA fragments separated on an agarose gel can be visualised after staining with
EtBr-stained DNA fluoresces orange under UV.
Ethidium bromide is used to stain DNA fragments, which appear as orange-coloured bands under UV light.
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