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Biology
Biotechnology: Principles and Processes
132 questions from 36 papers, each with its answer.
- 132 questions
- 36 papers
- 3.74% of Biology
Restriction Enzymes
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Which of the following is a restriction endonuclease?
Hind II was the first restriction endonuclease isolated.
Restriction endonucleases are 'molecular scissors' which cut DNA at specific sites. $EcoRI$ and $Hind$ II are examples.
Cloning vectors - plasmids
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Which of the following is not a feature of the plasmids?
Plasmids are double-stranded circular DNA.
A plasmid is extrachromosomal, double-stranded, circular DNA of bacteria. So 'single-stranded' is not a feature of plasmids.
PCR – Taq polymerase
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The $Taq$ polymerase enzyme is obtained from
Taq polymerase comes from $Thermus\ aquaticus$.
$Thermus\ aquaticus$ is a thermophile living in hot springs. 'T' and 'aq' of $Taq$ come from $Thermus\ aquaticus$. Taq DNA polymerase is used in PCR to replicate DNA.
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