Looking for classes? Ksquare Career Institute, Bengaluru →
Biology
Biotechnology: Principles and Processes
132 questions from 36 papers, each with its answer.
- 132 questions
- 36 papers
- 3.74% of Biology
Somatic Hybridization Technique
-
Arrange the following steps of somatic hybridisation in a correct sequence. I. Digestion of cell walls. II. Isolation of naked protoplasts. III. Fusion of protoplasts to get hybrid protoplast. IV. Isolation of single cells from two different varieties of plants. V. Growing of hybrid protoplast to form a new plant. Choose the CORRECT answer from the options given below:
Restriction endonucleases
-
Which of the following statements are NOT true regarding restriction endonucleases? I. They are called molecular scissors. II. These are the enzymes responsible for restricting the growth of bacteriophages in E. coli. III. They cut the DNA only at the centre of the palindromic sites. IV. They remove nucleotides only from the ends of DNA fragments. V. They recognise specific palindromic base-pair sequences. Choose the answer from the options given below:
Vectors and tools in biotechnology
-
Match List I with List II:
Pick the matching item from Column II for each row.
Column I
- i. Genetically modified organism
- ii. Thermostable DNA polymerase
- iii. Ti plasmid
- iv. pBR322
Column II
- a. $Agrobacterium\ tumefaciens$
- b. Bt cotton
- c. $Thermus\ aquaticus$
- d. $Escherichia\ coli$
Correct answer: i → b, ii → c, iii → a, iv → d
Polymerase Chain Reaction (PCR) steps
-
Identify the CORRECT sequence of steps in each cycle of Polymerase Chain Reaction:
Gel electrophoresis and DNA visualization
-
Which of the following statements are correct with respect to DNA separation, isolation and visualization? I. The cutting of DNA is done by molecular scissors. II. The DNA fragments separate according to their size in an agarose gel, upon electrophoresis. III. The separated DNA fragments can be seen without staining when exposed to UV light. IV. The separated DNA fragments, when stained with ethidium bromide, can be seen in visible light. Choose the CORRECT answer from the options given below:
pBR322 vector - restriction sites and antibiotic resistance genes
-
Insertion of a foreign DNA at $Bam$HI site in an $E.\ coli$ cloning vector pBR322 results in the loss of antibiotic resistance towards:
That is every question in Biotechnology: Principles and Processes for this filter.
Show all 132 questions Next: Biotechnology and its Applications
Want a score?
Answers here are not saved. Practise as a test to be marked and see your progress.
Practise as a test