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Biology
Biotechnology: Principles and Processes
132 questions from 36 papers, each with its answer.
- 132 questions
- 36 papers
- 3.74% of Biology
Techniques and instruments in biotechnology
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Match the following techniques or instruments with their usage:
Pick the matching item from Column II for each row.
Column I
- a. Bioreactor
- b. Electrophoresis
- c. PCR
- d. ELISA
Column II
- i. Separation of DNA fragments
- ii. Production of large quantities of products
- iii. Detection of pathogen, based on antigen-antibody reaction
- iv. Amplification of nucleic acids
Correct answer: a → ii, b → i, c → iv, d → iii
Bioreactor is used for production of large quantities of products (a-ii); Electrophoresis is used for separation of DNA fragments (b-i); PCR is used for amplification of nucleic acids (c-iv); ELISA is used for detection of pathogen based on antigen-antibody reaction (d-iii).
Gel electrophoresis - separation of DNA fragments
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In a mixture, DNA fragments are separated byGel electrophoresis separates DNA fragments by size, using an electric field to move negatively charged DNA fragments through a gel matrix, with smaller fragments moving faster/farther than larger ones.
Role of antibiotic resistance genes
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In Recombinant DNA technology antibiotics are usedIn this context, antibiotics are used to keep the culture medium free from unwanted/contaminating bacteria, while antibiotic resistance genes act as selectable markers to differentiate transformed from non-transformed cells.
Discovery of restriction endonucleases
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First discovered restriction endonuclease that always cuts DNA molecule at a particular point by recognising a specific sequence of six base pairs isHind II was the first restriction endonuclease discovered, found to always cut DNA molecules at a particular point by recognising a specific sequence of six base pairs (a palindromic sequence).
Restriction enzyme digestion
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Select the correct statement from the followingRestriction enzyme digestion involves incubating purified DNA molecules with the appropriate restriction enzymes under optimum reaction conditions (temperature, buffer, etc.) to cut the DNA at specific sites.
DNA isolation - spooling
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Spooling isSpooling refers to the process of collecting precipitated/isolated DNA, which appears as fine threads, typically by winding it around a glass rod after precipitation with chilled ethanol.
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